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Fig. 1.

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Localization of Golgi-resident proteins. (A) Localization of Golgi marker proteins with the cis-Golgi-resident marker Mnn9–mCherry. Wild-type cells expressing GFP-tagged Sed5 (cis), Gnt1 (medial), Sys1 (trans) and Sec7 (trans) with Mnn9–mCherry were grown to a mid-logarithmic phase in synthetic medium at 30°C and observed by 3D confocal fluorescence microscopy. Dashed lines indicate the edge of the cells. (B) Bar graph showing the percentage of Mnn9–mCherry-positive cisternae containing GFP-tagged Golgi-resident proteins. Error bars indicate s.d. from 10 independent cells. (C) Images of indicated areas in A. Scale bars: 1 µm.

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