FIG 6

Antibodies against MHC I inhibit JEV infection. Neuro2a cells were mock treated or incubated with 100 μg/ml rabbit IgG or MHC-I antibody alone or in combination with GRP78 C- and N-terminus-specific rabbit antibodies on ice for 1 h, followed by JEV infection at an MOI of 1. Viral RNA levels (A) and titers (B) were determined at 24 h p.i. The level of JEV RNA in mock-treated cells was taken as 1 for determining the relative RNA levels. The JEV RNA levels and viral titers were compared to those in mock-treated cells (**, P < 0.01). Each experiment contained biological duplicates, and the data presented are from three independent experiments. The error bars indicate SD.