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Published online 2017 Feb 20. doi: 10.18632/oncotarget.15521
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Tightly compacted spheroids show strong resistance to sorafenib compared to loosely compacted aggregates

A. HCC spheroids were formed for 3 days and treated with 10 μM of sorafenib for another 4 days. B. Cell viability was measured with resazurin assay. After 5 hr of treatment with 50 μM resazurin, absorbance was measured using an Enspire plate reader. The values were normalized to control (0.01% DMSO). Data represent the mean values ± SD from three independent experiments. All bright-field images of spheroids were obtained at 7 days using the same method. *P<0.05, **P<0.005. Scale bar = 200μm.

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