Fig. 3
Mitochondrial ROS production is affected by hypoxia and mitochondrial defects. NIH 3T3-scr, MECR-KD, HepG2-scr, MnSOD-KD, Mpv17+/+, and Mpv17-/- cells were cultured under normoxia (16% O2) and hypoxia (1% O2) for 20 h. The redox state was determined by measuring (A) DCF fluorescence, and (B) DHE fluorescence. * significant difference 16% O2 versus 1% O2, # significant difference NIH 3T3-scr versus MECR-KD, Mpv17+/+ versus Mpv17-/-, and HepG2-scr versus MnSOD-KD at 16% O2; p<0.05.
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