Figure 1.

AKAP effect on PKA cellular distribution and PKA activity at the plasma membrane in sensory neurons. A, immunohistochemical co-expression of PGP9.5 (red) and PKA RII (green) in TG and DRG cultured from naive AKAP WT and AKAP KO littermates. Yellow scale bar, 25 μm. PKA RII distribution of representative cells is shown in the right column: second order smoothing polynomial (green) of six individual plot profiles measured with 60° separations (gray) near the cell membrane (red). Confocal images are representative of three independent trials. B, PKA RII densitometry measurements taken from whole cell (left y axis) and plasma membrane (right y axis, black bars) of TG and DRG from AKAP WT (dark gray) and AKAP KO (red). n = 41–77 neurons/group. C, PKA RII plasma membrane/whole cell densitometry ratios from AKAP WT (gray) and AKAP KO (red) TG and DRG (from B). n = 41–77 neurons/group; *, p < 0.05; ***, p < 0.005; two-way ANOVA with Bonferroni post-hoc test. D, PKA activity at the plasma membrane in homogenized TG from AKAP WT (gray) and AKAP KO (red) mice. n = 3 independent trials performed in triplicate. ***, p < 0.005; unpaired Student's t test. E, PKA activity at plasma membrane in rat TG cultures treated in mock fashion (gray) or with AKAP siRNA (red). n = 3 independent trials performed in triplicate; **, p < 0.01; unpaired Student's t test. Error bars, S.E.