A mouse model for study of systemic HIV-1 infection, antiviral immune responses, and neuroinvasiveness

Mary Jane Potash; Wei Chao; Galina Bentsman; Nicolae Paris; Manisha Saini; Jadwiga Nitkiewicz; Paula Belem; Leroy Sharer; Andrew I. Brooks; David J. Volsky

doi:10.1073/pnas.0500649102

Fig. 4.

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EcoNDK infects conventional mice and alters cellular gene expression in the brain. (A) PCR amplification of EcoNDK viral DNA in spleen and brain 3 wk after inoculation; standard curves of amplification of plasmid DNA are at the right. (B) Quantitative real-time RT-PCR was conducted on total cellular RNA from brain tissue of EcoNDK 8 and 9, comparing levels of transcripts with levels found in the control brain. *, Differences compared with control at P < 0.05 by t test. (C) Immunocytochemical staining for STAT-1 in mouse cortical brain sections; arrows indicate examples of more intense staining for STAT-1 in NDK 8 (Right) compared with the control brain (Left). (×360.)