Fig. S1.

An external file that holds a picture, illustration, etc. Object name is pnas.1705406114sfig01.jpg

The role of ROS in mitochondrial calcium buffering capacity and membrane potential in the BAT of BATI4KO mice. (A) qRT-PCR of Complex I gene expression in BAT from WT and UCP1-KO mice housed at 4 °C or 30 °C; n = 5 mice per genotype. (B) qRT-PCR of Complex II gene expression in BAT from WT and UCP1-KO mice housed at 4 °C or 30 °C; n = 5 mice per genotype. (C) qRT-PCR of Complex III gene expression in BAT from WT and UCP1-KO mice housed at 4 °C or 30 °C; n = 5 mice per genotype. (D) qRT-PCR of Complex IV gene expression in BAT from WT and UCP1-KO mice housed at 4 °C or 30 °C; n = 5 mice per genotype. (E) qRT-PCR of ATP Synthase gene expression in BAT from WT and UCP1-KO mice housed at 4 °C or 30 °C; n = 5 mice per genotype. Data are presented as mean ± SEM. *P < 0.05; ***P < 0.01.

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