PMC full text:
Published online 2017 Jul 31. doi: 10.1371/journal.ppat.1006546
Fig 1
CoV-S mediated entry into tetraspanin KO cells.
(A) Western blot analysis of 293T and HeLa clonal cell lines. Actin and the tetraspanin CD63 are used as loading controls. (B) Immunofluorescent analysis of HeLa clonal cell lines. Unpermeabilized cells were incubated with primary antibodies against CD9, CD81 or CD63 as indicated. 293T WT or CD9KO cells were transfected with the appropriate receptors and CD9 where indicated. These cells were transduced with viruses pseudotyped with S proteins from MERS (C), 229E (D), SARS (E), or MHV (F). Pseudovirus transduction was measured by luciferase assay.