Fig. 1.

EM micrographs of frontal sections of Drosophila SCs (A–D) and a polycomplex (E) labeled with antibodies to the N-terminal domain of C(3)G (affinity-purified rabbit polyclonal) (A), the C-terminal end of the coiled-coil of C(3)G (mAb 1G5) (B), the C-terminal globular domain of C(3)G (mAb 1A8) (C), or the HA tag on the N terminus of C(2)M (mAb 3F10) (D and E). Some of the dark gold/silver particles are indicated by arrowheads. Although the light formaldehyde fixation necessary for immunolabeling is not as good for cellular preservation as are traditional EM fixation techniques, the basic structure of the SC is retained in these preparations (compare with Fig. 3A). (Scale bar, 100 nm.)