Figure 1

Cells were cultured in hydrogels of varying stiffness before treatment with doxorubicin. The experimental protocol is outlined in (A). Briefly, cells were seeded onto tissue culture plastic or into hydrogels that ranged in stiffness from 200 to 2,000 Pa. After 6 days in culture, samples were exposed to doxorubicin for 48 h and cell viability was determined using AOPI staining. Hydrogel stiffness was determined by calculating Young's modulus from frequency sweep measurements obtained from a rheometer (B).