Identification of functionally distinct fibro-inflammatory and adipogenic stromal subpopulations in visceral adipose tissue of adult mice

Chelsea Hepler; Bo Shan; Qianbin Zhang; Gervaise H Henry; Mengle Shao; Lavanya Vishvanath; Alexandra L Ghaben; Angela B Mobley; Douglas Strand; Gary C Hon; Rana K Gupta

doi:10.7554/eLife.39636

Figure 3—figure supplement 4.

An external file that holds a picture, illustration, etc.
Object name is elife-39636-fig3-figsupp4.jpg

Gonadal PDGFRβ+ Zfp423^GFP-High cells enrich for markers of committed preadipocytes.

(A) mRNA levels of endogenous Zfp423 in APCs, FIPs, and MLCs, isolated from gonadal WAT of 8-week-old male Zfp423^GFP mice. (B) Representative FACS gating strategy for the isolation of Zfp423^GFP-Low and Zfp423^GFP-High PDGFRβ+CD31 CD45- cells from gonadal WAT. (C) Pparg expression in Zfp423^GFP-Low and Zfp423^GFP-High cells isolated from gonadal WAT. (D) mRNA levels of Cluster 1A genes (APCs) in Zfp423^GFP-Low and Zfp423^GFP-High cells. (E) mRNA levels of Cluster 1B genes (Committed Preadipocytes) in Zfp423^GFP-Low and Zfp423^GFP-High cells. (F) mRNA levels of Cluster 2 genes (FIPs) in Zfp423^GFP-Low and Zfp423^GFP-High cells. (G) mRNA levels of Cluster 3 genes (MLCs) in Zfp423^GFP-Low and Zfp423^GFP-High cells. * in all graphs denote p<0.05 by student’s t-test. All bars represent mean +SEM. n = 4.