Figure 8.

(A) Distribution of Nr4a1, Nr4a2, and Nr4a3 expression, within tSNE plot depicted in Figure 1B. Transcript counts represent Log₂ of gene expression. (B) Nr4a mRNA levels in freshly isolated APCs, FIPs, and MLCs, isolated from the gonadal WAT of lean chow-fed male mice. n = 4. (C) Nr4a mRNA levels in freshly isolated APCs, FIPs, and MLCs, isolated from the gonadal WAT of male mice following 4 weeks of high-fat diet (HFD) feeding. n = 4. (D) Relative mRNA levels of Nr4a family members in cultures of FIPs treated with vehicle (PBS) or TNFα (20 ng/ml) for 3 hr. n = 4. * denotes p<0.05 by student’s t-test. n = 4. (E) Relative mRNA levels of Nr4a1 in FIPs 3 days following transduction with retrovirus expressing either Gfp or Nr4a1. n = 4. (F) Relative mRNA levels of Nr4a2 in FIPs 3 days following transduction with retrovirus expressing either Gfp or Nr4a2. n = 4. (G) Relative mRNA levels of Nr4a3 in FIPs 3 days following transduction with retrovirus expressing either Gfp or Nr4a3. n = 4. (H) Cxcl2 expression in FIPs 3 days following transduction with indicated retroviruses and treated with vehicle (PBS) or TNFα (20 ng/ml) for 4 hr. n = 4. (I) Cxcl10 expression in same cultures shown in (H). (J) Ccl2 expression in same cultures shown in (H). (K) Il6 expression in same cultures shown in (H). (L) Relative mRNA levels of Nr4a1 in FIPs following transduction with retrovirus expressing shRNA targeting Gfp (shGFP) (control) or retroviruses individually expressing distinct shRNAs targeting unique regions of Nr4a1 mRNA (shNr4a1 #1–3). n = 4. (M) Ccl2 expression in FIPs following transduction with indicated retroviruses and treatment with vehicle (PBS) or TNFα (20 ng/ml) for 3 hr. n = 4. (N) Tnfa expression in same cultures shown in (C). (O) Cxcl2 expression in same cultures shown in (C). (P) Il6 expression in same cultures shown in (C). * in panels E-P denote p<0.05 by student’s t-test in comparison to corresponding treatments of control cells (pMSCV-GFP or shGFP). Bars in all graphs represent mean +SEM.