Figure 5.

fmn mutants have attenuated rest rebound after rest deprivation. The plots for w (a) and fmn (b) show changes in the amount of rest during a 24 h period of time. Rest % is the fraction of 5 min intervals in each 2 h time period with no activity. Rest deprivation was initiated at Zeitgeber time 16, 18, or 20, representing 6, 4, or 2 h of deprivation. Control populations in these experiments were flies of the same genotype that were not disturbed during the 24 h period. For wild-type flies in a, the differences between treated and control flies were significant (two-way ANOVA; p < 0.05) for all three periods of deprivation (2, 4, or 6 h), both during and after deprivation at CT 0 (^*). For fmn flies, differences between treated and control flies were significant during deprivation (at CT 16, 18, and 20), but there was no significant rebound observed after deprivation. Error bars indicate the SEM (n = 15 for all time points). This figure shows the results of one of two experiments that yielded almost identical results.