Figure 4.

Single-cell PCR indicates the presence of mRNA for KCNQ2,KCNQ3, and KCNQ5, but not KCNQ4, in most SCG neurons tested at P17 and P45. Gels show bands from eight cells from P17 rats (A) and seven cells from P45 rats (B) using the primers described in Materials and Methods. Bands generated from the individual KCNQ cDNA plasmids are shown in the lanes marked P in B. No signals were detected in the absence of cDNA templates. M, Marker ladder.