Figure 2

Let-7f-5p promotes osteogenic differentiation of BMSCs by targeting TGFBR1 under Dex conditions. (A) Increased let-7f-5p expression in BMSCs transfected with let-7f-5p mimics. Results are shown as mean ± s.d. of three independent experiments performed in triplicates, ^***P < 0.001 by t test. (B-C) ALP staining and Von Kossa staining of BMSCs transfected with let-7f-5p mimics with Dex treatment. (D) Quantitative analysis of the ALP activity in BMSCs transfected with let-7f-5p mimics with Dex treatment. Results are shown as mean ± s.d. of three independent experiments performed in triplicates, ^***P < 0.001 by t test. (E) Increased mRNA expression of Runx2, Osx, Alp, and Ocn in BMSCs transfected with let-7f-5p mimics with Dex treatment. Results are shown as mean ± s.d. of three independent experiments performed in triplicates, ^*P < 0.05 and ^**P < 0.01 by t test. (F-H) let-7f-5p inhibited mRNA expression and protein expression of TGFBR1 in BMSCs under Dex conditions. Results are shown as mean ± s.d. of three independent experiments performed in triplicates, ^**P < 0.01 and ^***P < 0.001 by t test.