Figure 5

The CSMD1-3′-UTR is a target of miR-10b. The diagram of the luciferase reporter plasmids: plasmid with the full length wild-type CSMD1-3′-UTR (pMIR- CSMD1-3'-UTR-WT) insert and plasmid with a mutant CSMD1- 3′-UTR (pMIR-CSMD1-3'-UTR-mut) which carried a substitution of seven nucleotides (TGTCCCA) within the miR-10b binding site (707-713). Luciferase activity assay demonstrates a direct targeting of the 3′-UTR of CSMD1 by miR-10b. HGC27 and SGC7901 cells were transfected with pcDNA3.1-miR-10b and pMIR-CDMD1-3′- UTR-WT or pMIR-CSMD1-3′-UTR-mut. (A) HGC27 cells; (B) SGC7901 cells.