HLA-A∗0201 T-cell epitopes in severe acute respiratory syndrome (SARS) coronavirus nucleocapsid and spike proteins

Yeou-Ping Tsao; Jian-Yu Lin; Jia-Tsrong Jan; Chih-Hsiang Leng; Chen-Chung Chu; Yuh-Cheng Yang; Show-Li Chen

doi:10.1016/j.bbrc.2006.03.152

Fig. 1

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Stabilization of HLA-A^∗0201 molecules on the surface of T2-cells by computer predicted peptides of SARS-CoV N and S protein. The HLA-A2.1 expression was determined by FACS staining with the BB7.2 mAb, and the median fluorescence intensity calculated, respectively. (A) T2-cells were incubated with N peptides and (B) T2-cells were incubated with S peptides. Three independent experiments were involved in each column and the summarized data shown in the histogram. YMDG, the HLA-A2.1 tyrosinase-derived peptide YMDGTMSQV served as a positive control. EADP, the HLA-A1-binding peptide EADPTGHSY (MAGE-1) served as a negative control.