PMC full text:
Published online 2013 Mar 13. doi: 10.1038/nature12005
Figure 2
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hCoV-EMC S1 binding to DPP4.
a, Huh-7 cell lysates were incubated with hCoV-EMC and SARS-CoV S1–Fc proteins and affinity-isolated proteins were subjected to protein electrophoresis under non-reducing conditions. The arrowhead indicates the position of the ∼110-kDa DPP4 protein specifically isolated using the hCoV-EMC S1–Fc protein. b, hCoV-EMC and SARS-CoV S1–Fc proteins were mock-incubated or incubated with soluble DPP4 (sDPP4) or soluble ACE2 (sACE2) followed by protein A sepharose affinity isolation and subjected to protein electrophoresis under non-reducing conditions.