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Fig. 2
Real-time RT-PCR for SARS-CoV RNA. Vero E6 cells had been treated with nelfinavir or ritonavir for 3 h before infection at the concentration of 10 μM. Instead of these drugs, PBS was added as a negative control. Viral RNA in the culture supernatant was collected 24 h after infection and quantified by the use of a fluorogenic probe. All samples were analyzed in triplicate.
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