Papain-like protease regulates SARS-CoV-2 viral spread and innate immunity

Donghyuk Shin; Rukmini Mukherjee; Diana Grewe; Denisa Bojkova; Kheewoong Baek; Anshu Bhattacharya; Laura Schulz; Marek Widera; Ahmad Reza Mehdipour; Georg Tascher; Paul P. Geurink; Alexander Wilhelm; Gerbrand J. van der Heden van Noort; Huib Ovaa; Stefan Müller; Klaus-Peter Knobeloch; Krishnaraj Rajalingam; Brenda A. Schulman; Jindrich Cinatl; Gerhard Hummer; Sandra Ciesek; Ivan Dikic

doi:10.1038/s41586-020-2601-5

Extended Data Table 3

Binding kinetics of PLpro to K48-Ub₂ or ISG15

		k_on ± S.E.M^a	k_off S.E.M^a	k_d ± S.E.M^a	R^2b
		(10²M^-1s^-1)	(10^-1 s^-1)	(μM)	R^2b
CoV2	Triazole linked K48-Ub₂	158 ±8.00	4.30 ±0.11	27.28 ±1.55	0.99
PLpro	HumanISG15	1530 ±52.4	2.15 ±0.06	1.41 ±0.06	0.99
	MouseISG15	122 ±4.48	0.20 ± 0.02	1.64 ±0.17	0.97
SARS PLpro	Triazole linked K48-Ub₂	820 ±32.1	0.81 ±0.02	0.99 ± 0.04	0.98
	HumanISG15	88.8 ±14.5	0.76 ± 0.06	8.60 ±1.56	0.98
	MouseISG15	24.7 ± 0.75	0.23 ± 0.01	9.34 ±0.41	0.98

Binding kinetic parameters for SCoV-PLpro, SCoV2-PLpro to triazole-linked K48-Ub2, humanISG15 or mouseISG15 are presented. Values are presented as mean ± s.e.m. (n = 3, independent experiments).

^as.e.m.

^b R ², goodness of the curve fit between experimental data and mathematical 1:1 binding curve