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Published online 2020 Apr 28. doi: 10.1186/s12870-020-02391-7
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Fig. 8

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Expression analysis of anthocyanin biosynthesis genes in stem, leaf and flower from two EsMYB90 transgenic tobacco lines at the flowering stage. WT: Wild Type; L2 and L4: two independent 35S:EsMYB90 lines. Ntubi2 (Ntubiquitin 2, LOC107772211) was used as an internal control. (a) NtPAL (XM_016625506.1); (b) NtCHS (NM_001326166.1); (c) NtCHI (NM_001325287.1); (d) NtF3H (NM_001325083.1); (e) NtF3’H (NM_001325608.1); (f) NtDFR (NM_001325732.1); (g) NtLDOX (NM_001326043.1); (h) NtUFGT (NM_001325312.1). The ΔΔCt method was used to determine the relative expression, and expression levels of tested genes in the wild type were set to 1. Vertical bars represent standard errors of three biological replicates. b and c indicate p < 0.01 and p < 0.001, respectively

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