A novel rapid detection for SARS-CoV-2 spike 1 antigens using human angiotensin converting enzyme 2 (ACE2)

Jong-Hwan Lee; Minsuk Choi; Yujin Jung; Sung Kyun Lee; Chang-Seop Lee; Jung Kim; Jongwoo Kim; Nam Hoon Kim; Bum-Tae Kim; Hong Gi Kim

doi:10.1016/j.bios.2020.112715

Fig. 4

Identification of the sandwich pair for detection of SARS-CoV-2 spike antigen. a) Schematic diagram of LFIA using ACE2 as the capture probe and sandwich analysis results obtained from paired antibodies (CR3022, F26G19, and S1mAb). SARS-CoV-2 S1 antigen (50 ng) was used as a positive control, and buffer containing no S1 antigen was used as a negative control. After 20 min, the strips were captured by a smartphone, and their peak intensities were analyzed. b) Schematic diagram of LFIA, using antibodies as the capture probe, and their sandwich analysis results. c) Peak intensities of capture probe (P_C)–detection probe (P_D) pairs. A total of 12 pairs of positive controls (50 ng S1 antigen) were tested, and their intensities were analyzed. Peak intensity was calculated by subtracting the background intensity of the strip from the average intensities of the dots.