Figure 1

(A) Experimental setup has mice with a chronically implanted optode and electrode in their barrel cortex. Awake freely moving mice received one 0.2-second MES per day for 3 days with concurrent local field potential and local partial pressure of oxygen (pO₂) recordings. (B and C) Mean oxygen profile before, during, and after MES in male (B) and female (C) C57BL/6J mice (N = 5, each). The inset shows the duration of electrographic seizures during the 3 seizures. (D) Quantification (mean ± SEM) of the area (depth and duration) below the severe hypoxic threshold (pO₂ < 10 mmHg) following the third seizure. Male and female C57BL/6J mice were not different from each other (t test, t[8] = 1.11, P = 0.30). (E and F) Mean oxygen profile before, during, and after MES in mixed sex Slc1a3 (astrocyte reporter) (E) and PdgfrB (mural cell reporter) (F) mice (N = 5, each). (G) Quantification (mean ± SEM) of the area below the severe hypoxic threshold (pO₂ < 10 mmHg) following the third seizure. Slc1a3 and PdgfrB and combined male and female C57BL/6J mice were not different from each other (1-way ANOVA, F[2,17] = 0.56, P = 0.58).