MYOGLOBIN PROMOTES NITRITE-DEPENDENT MITOCHONDRIAL S-NITROSATION TO MEDIATE CYTOPROTECTION AFTER HYPOXIA/REOXYGENATION

Kelly Quesnelle; Danielle A. Guimaraes; Krithika Rao; Anuradha Bharara Singh; Yinna Wang; Neil Hogg; Sruti Shiva

doi:10.1016/j.niox.2020.08.005

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Myoglobin expression enhances nitrite-dependent attenuation of cell death after hypoxia/reoxygenation.

(A) Cell death (percent of lactate dehydrogenase release) in CHO-R (open circles) and CHO-Mb (filled squares) cells after hypoxia/reoxygenation with nitrite treatment (0–100μM). (B) Mitochondrial complex I activity in CHO-R (open bars) and CHO-Mb (filled bars) cells in normoxia (control), after nitrite (25μM) treatment in normoxia (Nitrite), after hypoxia/reoxygenation (H/R), or after hypoxia/reoxygenation in the presence of nitrite (25μM; H/R + Nitrite). (C) Hydrogen peroxide production by CHO-R (open bars) and CHO-Mb (filled bars) cells in normoxia (Control), after hypoxia/reoxygenation (H/R), or after hypoxia/reoxygenation in the presence of nitrite (25μM; H/R + Nitrite). (D) S-nitrosothiol levels of mitochondria isolated from CHO-R and CHO-Mb cells in normoxia (control) after hypoxia/reoxygenation (H/R), or hypoxia/reoxygenation in the presence of nitrite either 10 minutes (10m) or 2 hours (2h) after reoxygenation. *p<0.01; #p<0.05. Data are means ± SEM. n=5 for all panels.