Figure 1.
Schematic Diagram of Basic Steps in Droplet-Based Microfluidic scRNAseq.
Cells and DNA-barcoded beads are partitioned into a microfluidic device and mixed with oil to generate nanoliter-sized single cell droplets. Each bead contains primers, cellular barcodes, and a unique molecular identifier (UMI). Cells are lysed within droplets, and reverse transcription either takes place within the droplets or following primer annealing and demulsification. Downstream steps differ slightly between platforms (not shown) to generate libraries for sequencing. Following sequencing, reads are demultiplexed, aligned, and then UMI counts per cell are quantified. Modified from (78).