Figure 2.
Integrated scRNAseq of Immune-Depleted Stromal Cells Isolated from eWAT of Adult Mice.
Publicly available scRNAseq datasets (64, 104) from immune-depleted stromal cells isolated from eWAT were integrated using Seurat (105). (A) tSNE plots displaying the overlay of integrated datasets (left) and cell cluster identification (right). (B) tSNE plots displaying the log2 expression levels for select genes commonly used to purify adipose stem cells by FACS and/or used in lineage tracing experiments. (C) Violin plots displaying relative log2 expression levels of select marker genes in individual clusters. Abbr: Ly6a (stem cell antigen-1 (Sca-1)), Itgb1 (CD29).