Figure 2

SW480 and SW620 cells have different expression levels of ORAI1 and EMT-relevant markers. (A) Western blot analysis of ORAI1 and EMT-relevant markers expression in these two CRC cell lines. (B) The metastatic capability of these two cell lines was detected by transwell migration and invasion assays. magnification, ×200. (C) Immunofluorescence was performed to evaluate the expression of E-cadherin and Vimentin in these two CRC cell lines, Scale bar 75μm. All the experiments were repeated three times. *P<0.05; **P<0.01.