Lentiviral vector induces high-quality memory T cells via dendritic cells transduction

Min Wen Ku; Pierre Authié; Fabien Nevo; Philippe Souque; Maryline Bourgine; Marta Romano; Pierre Charneau; Laleh Majlessi

doi:10.1038/s42003-021-02251-6

Fig. 3

Capacity of all major APCs subsets transduced in vivo by lentiviral vector harboring β2m promoter to activate CD8⁺ T cells.

a C57BL/6 mice (n = 6/group) were immunized i.m with 5 × 10⁷ TU of LV-β2m-GFP or LV-β2m-OVA. Shown are representative cytometric plots for pLN immune cells from an LV-β2m-GFP or an LV-β2m-OVA (negative control) immunized mouse, as analyzed at 5 dpi to detect GFP⁺ immune cells transduced in vivo by LV harboring β2m promoter. b Percentage of GFP⁺ cells in the respective immune cell subsets expressed as mean ± SEM. Dots represent biological replicates. c Sorting strategy for purification of immune cell populations from popliteal lymph nodes of C57BL/6 mice (n = 3/group) immunized i.m with 5 × 10⁷ TU of LV-β2m-GFP or LV-β2m-OVA. d CFSE-labeled OT1 CD8⁺ T cells were cultured for 2 days with the indicated immune cell subsets sorted from C57BL/6 mice immunized with 5 × 10⁷ TU of LV-β2m-OVA or LV-β2m-GFP (negative control). The activation and cell division of OT1 CD8⁺ T cells was indicated by the dilution of covalently bound CFSE divided into the daughter cells, as compared between LV-β2m-OVA and LV-β2m-GFP. For (a), (b), (c), and (d), pDCs, plasmacytoid dendritic cells; cDCs, conventional dendritic cells; Mφ, macrophages.