FIG. 4

(A) The promoter of RPL30. The start codon is boxed. The two Rap1p binding sites are indicated by a heavy underline, and the two T-rich regions are indicated by a light underline. The initiation of transcription, termed +1 and marked with an arrow, is 58 nucleotides upstream of ATG. (B) The RPL30-ACT1 fusion gene. See Materials and Methods for details. (C) Constructing the fused gene with the ACT1 promoter driving the RPL30 transcript (constructs A to D); see Materials and Methods. The stippled area represents sequences from ACT1. Nucleotides from the RPL30 promoter were fused to the ACT1 TATA region to form constructs B, C, and D. The hatched boxes are ACT1 sequences; the open boxes are RPL30 sequences; the black boxes represent the RPL30 Rap1p binding sites. The line represents the L30 transcript. The nucleotide boundaries of the RPL30 sequences are shown above the constructs, and those of the ACT1 sequences are shown below the constructs. Because the ACT1 gene has multiple sites of transcription initiation, the numbering is in reference to ATG of the coding region.