The Protective Effect of Ubiquinone against the Amyloid Peptide in Endothelial Cells Is Isoprenoid Chain Length-Dependent

Javier Frontiñán-Rubio; Yoana Rabanal-Ruiz; Mario Durán-Prado; Francisco Javier Alcain

doi:10.3390/antiox10111806

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Evaluation of the energetic cellular phenotype in cells exposed to Aβ_25–35 and different CoQs. bEnd.3 cells were pretreated for 24 h with CoQ2, CoQ6, CoQ9, or CoQ10 and then treated with Aβ_25–35 for 24 h. (A) OCR levels are expressed as oxygen consumption (picomoles) per minute. (B) ECAR levels are expressed as changes in pH per minute. Results were normalized against the total number of cells per well. Data are presented as the mean ± SEM. Student’s t-test was used to compare the differences between groups treated with the same ubiquinone and Dunnett’s multiple comparison test to compare each Aβ_25–35 (plus or minus ubiquinone) treatment with the control. ns: not significant, * p < 0.05, ** p < 0.01 (n = 3).