Figure 5

Real-time characterization of Aβ_25–35 internalization and mitochondrial dynamics. bEnd.3 cells were pretreated with CoQ2, CoQ6, CoQ9, or CoQ10 for 24 h and were then treated with Aβ_25–35 HyLite^TM 488. (A) Levels of fluorescent Aβ_25–35 were measured every two hours and normalized vs. 0 h of exposure. Images show internalized Aβ_25–35 after 10 h of exposure in individual cells (white line represents the cell outline) (scale bar = 20 µM). (B) Mitochondrial status was quantified as the intensity of Mitotracker CMXRos compared to 0 h of exposure to Aβ_25–35. Data are presented as the mean ± SEM. Dunnett’s multiple comparison test compared each Aβ_25–35 plus ubiquinone treatment with Aβ_25–35. ns: not significant, * p < 0.05, ** p < 0.01, *** p < 0.001, (>30 cells/condition).