Figure 6

Aβ_25–35 internalization and mitochondrial colocalization in bEnd.3 cells exposed to Aβ_25–35 and different CoQs. bEnd.3 cells were pretreated with CoQ2, CoQ6, CoQ9, or CoQ10 for 24 h and then treated with Aβ_25–35 HyLite^TM 488 for 24 h. (A) Internalized fluorescent Aβ_25–35 is expressed as RFUs. (B) Mitochondrial-fluorescent Aβ_25–35 was measured as the percentage of fluorescent Aβ_25–35 present into mitochondria compared to total internalized Aβ_25–35. (C) Images show cells stained with Mitotracker CMXros (red) and Aβ_25–35 (green) and their colocalization. Detailed images show the colocalized areas in white (scale = 20 µM/scale augmented = 5 µM). Data are presented as the mean ± SEM. Dunnett’s multiple comparison test compared each Aβ_25–35 plus ubiquinone treatment with Aβ_25–35. ns: not significant, * p < 0.05, ** p < 0.01, *** p < 0.001, (n = 3).