FIG. 9

Increased levels of cyclin D1 protein in cyclinD2-deficient CGNPs treated with Shh. Protein lysates (25 μg) of CGNP cultures from cyclinD2-deficient mice and their wild-type or heterozygous littermates were prepared after 24 h of treatment with Shh or vehicle and then analyzed by Western blot assay for cyclin D1 protein. Genotypes are indicated above the lanes. Note the equivalent responses of cyclin D1 protein levels, an indicator of cell cycle progression, to Shh in cyclinD2^−/− CGNPs.