Figure 9

Orai3 regulates pancreatic cancer metastasis. (A) Whole-body bio-fluorescence imaging signals at 12-week post-injection timepoint in the case of shNT Panc1 stables injected mice. (A’) Whole-body bio-fluorescence imaging signals in shOrai3 Panc1 stable cells injected mice at 12-week post-injection timepoint. The initial xenografts at the site of injections are marked in red circles while metastatic spread is highlighted with yellow arrows. (B) Thoracoabdominal region bio-fluorescence imaging signals at 12-week post-injection time-point in case of shNT Panc1 stables injected mice. (B’) Thoracoabdominal region bio-fluorescence imaging signals in shOrai3 Panc1 stable cells injected mice at 12-week post-injection timepoint. The thoracoabdominal bio-fluorescence imaging signals are identified in red circles. The time interval between whole body and thoracoabdominal bio-fluorescence imaging was 3 to 5 min. (C) Western blot analysis for MMP2 protein expression in shNT Panc1 and shOrai3 Panc1 tumors. (D) Densitometric analysis of MMP2 levels in shNT Panc1 and shOrai3 Panc1 tumors. (E) Western blot analysis for E-cadherin protein expression in shNT Panc1 and shOrai3 Panc1 tumors. (F) Densitometric analysis of E-cadherin levels in shNT Panc1 and shOrai3 Panc1 tumors. Data presented as mean ± S.E.M. Un-paired t-test was performed for statistical analysis. p-value < 0.05 was considered as significant and is presented as “*”.