Rutaecarpine Promotes Adipose Thermogenesis and Protects against HFD-Induced Obesity via AMPK/PGC-1α Pathway

Dandan Chen; Yanan Duan; Shuxiang Yu; Xinwen Zhang; Ni Li; Jingya Li

doi:10.3390/ph15040469

Figure 1

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Rut increases expression of PGC-1α and drives the thermogenesis program. (A) The screening result of Rut in activating PGC-1α. (B) Effect of Rut on activating PGC-1α in C3H10-T1/2. (C) RT-qPCR analysis of genes indicated in C3H10-T1/2. (D) Expressions of protein indicated in C3H10-T1/2 treated by Rut for 24 h. (E–G) Relative protein level of PGC-1α (E), UCP1 (F) and NRF2 (G) compared to β-actin. n = 3 per group. Data are presented as the means ±SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, Rut groups versus vehicle group by one-way ANOVA.