Figure 4

Rut promotes heat generation and energy expenditure in HFD-fed mice. (A) Monitoring of oxygen consumption (VO2). (B) Average O2 consumption. (C) Analysis of energy expenditure (EE) in 24 h and after administration of CL316,243. (D) Average energy expenditure. (E) Rectal temperatures of HFD mice were recorded every hour after transition to 4 °C from 22 °C. (F) Representative infrared thermal images of HFD-fed mice treated with vehicle or Rut after 6 h of exposure to 4 °C. (G) Quantification of the interscapular BAT skin temperatures of HFD mice. (H,I) RT-qPCR analysis of thermogenic genes in iBAT (H) and iWAT (I) from HFD mice. (J,K) Western blot analysis of proteins indicated in iBAT (J) and iWAT (K). n = 4–6 per group. Data are presented as the means ±SEM. Student’s t-test. * p < 0.05, ** p < 0.01 compared with the indicated control group.