Figure 5

Thermogenic effects induced by Rut treatment are ablated in thermoneutral conditions. (A,B) Body weights of HFD-fed mice treated with vehicle or Rut housed at 22 °C (A) or 30 °C (B). (C) Rectal temperature changes of mice were recorded every hour during cold stimuli. (D) Representative infrared thermal images of mice after cold exposure. (E) Quantification of interscapular BAT skin temperatures of 22 °C or 30 °C mice treated with vehicle or Rut. (F,G) The changes in O₂ consumption of mice acclimated at 22 °C (F) and 30 °C (G). (H) Assessment of average O₂ consumption. n = 5 per group. Data are presented as the means ±SEM. * p < 0.05, ** p < 0.01, Rut group versus vehicle group; # p < 0.05, ## p < 0.01, ### p < 0.001, 30 °C group versus 22 °C group by Student’s t-test.