Rutaecarpine Promotes Adipose Thermogenesis and Protects against HFD-Induced Obesity via AMPK/PGC-1α Pathway

Dandan Chen; Yanan Duan; Shuxiang Yu; Xinwen Zhang; Ni Li; Jingya Li

doi:10.3390/ph15040469

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Rut stimulates thermogenic program via AMPK/PGC-1α pathway. (A) Expression of PGC-1α and UCP1 protein by siRNA against PGC-1α. (B,C) Relative protein level of PGC-1α (B) and UCP1 (C) compared to β-actin. (D) The mRNA level of Pgc-1α and Ucp1 after blockage of PGC-1α. (E) Expression of p-AMPK, AMPK, p-ACC and ACC protein in C3H10-T1/2 treated by Rut. (F) Expression of PGC-1α and UCP1 protein in C3H10-T1/2 with siRNA against AMPK and Rut treatment. (G,H) Relative protein level of PGC-1α (G) and UCP1 (H) compared to β-actin. (I) The mRNA level of Pgc-1α and Ucp1 after silence of AMPK. n = 3 per group. Data are presented as the means ±SEM. * p < 0.05, ** p < 0.01, Rut group versus vehicle group; # p < 0.05, ## p < 0.01, ### p < 0.001, siRNA group versus control group by Student’s t-test.