Figure 7

Thermogenic effects induced by Rut are attenuated by absence of AMPK. (A) Body weight of the indicated mice group. (B) Rectal temperature changes of four groups mice were recorded at 4 °C. (C) Representative infrared thermal images of floxed or AKO mice treated with vehicle or Rut after 6 h of exposure to 4 °C. (D) Statistical graphs of interscapular BAT skin heat. (E) Glucose levels were plotted versus times post insulin injection. (F) Analysis of AUC during ITT. (G) Testing of O₂ consumption. (H) Average of O₂ consumption under basal and CL316,243 stimulation conditions. (I) Assessment of EE. (J) Average of EE under basal and CL316,243 stimulation conditions. (K,L) Expression level of thermogenic genes by RT-qPCR analysis in iBAT (K) and iWAT (L). (M,N) Western blot analysis of AMPK, PGC-1α and UCP1 expression level in iBAT (M) and iWAT (N) of different groups mice. n = 5–6 per group. Data are presented as the means ±SEM. Student’s t-test. * p < 0.05, ** p < 0.01, *** p < 0.001, Rut group versus vehicle group; # p < 0.05, ## p < 0.01, ### p < 0.001, AKO group versus floxed group.