Figure 7

(A) Schematic of flurothyl-induced seizure protocol. (B) Schematic of experimental paradigm for 8-day flurothyl seizure kindling and rechallenge. (C) Graphs of mean ± SEM latencies to myoclonic (top left) and generalized (top right) seizure depicting changes in seizure threshold throughout flurothyl kindling and rechallenge, analyzed by 2-way repeated-measures ANOVA, Tukey’s post hoc; and graph of median and range for generalized seizure severity based on a modified Racine scale (bottom), analyzed by mixed-effects generalized linear modeling (see Supplemental Figure 10 for details). Data represent mice surviving all 8 days of flurothyl kindling. (D and E) Group survival (D) and proportionality of seizure severity (E) for all tested mice. The left panel in E depicts the proportion of mice experiencing at least 1 seizure with tonic hind limb extension (THLE; score of 6 or 7 on modified Racine scale) versus the proportion not experiencing any (Non-THLE, score <6); the right panel depicts the proportion of mice in which THLE seizures progressed to death (score of 7). Survival curves were compared with the log-rank (Mantel-Cox) test. The proportionality of seizure severity was analyzed using χ² statistics. *P < 0.05, **P < 0.01, ****P < 0.0001.