TABLE 1
Bacterial strains and plasmids
| Strain or plasmid | Characteristics | Source or reference |
|---|---|---|
| S. pneumoniae | ||
| D39 | Type 2 encapsulated | 6, 59 |
| Rx1 | Nonencapsulated mutant of D39 | 48 |
| JY53 | pspA mutant of D39 | 59 |
| TRE118 | pspC mutant of D39 | This study |
| TRE121 | pspA pspC mutant of D39 | This study |
| WU2 | Type 3 encapsulated | 17 |
| EF3296 | Type 4 encapsulated | 2, 59 |
| L81905 | Type 4 encapsulated | 19 |
| EF3030 | Type 19F encapsulated | 1 |
| CCUG10175 | Type 19F encapsulated | 2 |
| M. catarrhalis | Clinical isolate | Clinical bacteriology lab, Lund, Sweden |
| E. coli M15 | Qiagen | |
| Plasmids | ||
| pHR101 | pQE40::BglII-HindIII fragment, encodes PspA/EF3296 (1–115) | This study |
| pHR102 | pQE40::SalI-SphI fragment, encodes PspA/EF3296 (75–305) | This study |
| pHR105 | pQE40::SalI-SphI fragment, encodes PspA/EF3296 (1–411) | This study |
| pHR107 | pQE40::SalI-SphI fragment, encodes PspA/EF3296 (75–490) | This study |
| JAS218 | PQE40::BamHI-SalI fragment, encodes PspA/Rx1 (167–288) | This study |
| PBAR4285 | Encodes PspA/Rx1 (1–115) | 38, 44, 59 |
| pBAR4310 | Encodes PspA/Rx1 (1–192) | 38, 44, 59 |
| pBAR501 | Encodes PspA/Rx1 (288–563) | 38, 44, 59 |
| pUAB55 | Encodes PspA/Rx1 (1–370) | 12, 19 |
| pUAB103 | Encodes PspA/Rx1 (1–303) | 19 |