Downregulation of Cav3.1 T-type Calcium Channel Expression in Age-related Hearing Loss Model
- PMID: 34403092
- DOI: 10.1007/s11596-021-2416-0
Downregulation of Cav3.1 T-type Calcium Channel Expression in Age-related Hearing Loss Model
Abstract
Objective: Age-related hearing loss (AHL), characterized by degeneration of cochlea structures, is the most common sensory disorder among the elderly worldwide. The calcium channel is considered to contribute to normal hearing. However, the role of the T-type voltage-activated calcium channel, Cav3.1, remains unclear in AHL. Here, we investigate the age-related change of Cav3.1 expression in the cochlea and D-gal-induced senescent HEI-OC1 cells.
Methods: Cochleae from C57BL/6 mice at 2 months and 12 months of age were assessed. Senescence in House Ear Institute-Organ of Corti 1 (HEI-OC1) cells was induced by D-gal treatment. The immunofluorescence technique was employed to investigate the distribution of Cav3.1 in vivo and in vitro. Quantitative assessment was achieved by Western blotting and real-time PCR.
Results: In comparison with 2-month-old animals, 12-month old C57BL/6 mice exhibited great loss of hair cells and elevated auditory brainstem threshold. The Cav3.1 was located in hair cells, spiral ganglion cells, lateral walls, and the expression of Cav3.1 protein and mRNA decreased in the aged cochleae. D-gal-induced senescence assay confirmed the down-regulation of Cav3.1 expression in senescent HEI-OC1 cells.
Conclusion: Our results show that age-related down-regulated expression of Cav3.1 in the cochleae is associated with AHL and may contribute to the pathogenesis of AHL.
Keywords: C57BL/6 mouse; HEI-OC1; cochlea; presbycusis.
© 2021. Huazhong University of Science and Technology.
Similar articles
-
Down-regulation of the large conductance Ca(2+)-activated K(+) channel expression in C57BL/6J cochlea.Acta Otolaryngol. 2016 Sep;136(9):875-8. doi: 10.3109/00016489.2016.1168941. Epub 2016 Apr 19. Acta Otolaryngol. 2016. PMID: 27093472
-
Age-related hearing loss and the ahl locus in mice.Hear Res. 2004 Feb;188(1-2):21-8. doi: 10.1016/S0378-5955(03)00365-4. Hear Res. 2004. PMID: 14759567 Free PMC article.
-
STK33 alleviates gentamicin-induced ototoxicity in cochlear hair cells and House Ear Institute-Organ of Corti 1 cells.J Cell Mol Med. 2018 Nov;22(11):5286-5299. doi: 10.1111/jcmm.13792. Epub 2018 Sep 6. J Cell Mol Med. 2018. PMID: 30256516 Free PMC article.
-
Mouse models of age-related mitochondrial neurosensory hearing loss.Mol Cell Neurosci. 2013 Jul;55:95-100. doi: 10.1016/j.mcn.2012.07.004. Epub 2012 Jul 20. Mol Cell Neurosci. 2013. PMID: 22820179 Free PMC article. Review.
-
Genetic influences on susceptibility of the auditory system to aging and environmental factors.Scand Audiol Suppl. 1992;36:1-39. Scand Audiol Suppl. 1992. PMID: 1488615 Review.
Cited by
-
Factors influencing the relationship between cochlear health measures and speech recognition in cochlear implant users.Front Integr Neurosci. 2023 May 12;17:1125712. doi: 10.3389/fnint.2023.1125712. eCollection 2023. Front Integr Neurosci. 2023. PMID: 37251736 Free PMC article.
References
-
- Wattamwar K, Qian ZJ, Otter J, et al. Increases in the Rate of Age-Related Hearing Loss in the Older Old. JAMA Otolaryngol Head Neck Surg, 2017,143(1):41–45 - DOI
-
- Hotka M, Cagalinec M, Hilber K, et al. L-type Ca(2+) channel-mediated Ca(2+) influx adjusts neuronal mitochondrial function to physiological and pathophysiological conditions. Sci Signal, 2020,13(618):eaaw6923 - DOI
-
- Yagami T, Kohma H, Yamamoto Y. L-type voltage-dependent calcium channels as therapeutic targets for neurodegenerative diseases. Curr Med Chem, 2012, 19(28):4816–4827 - DOI
-
- Benkert J, Hess S, Roy S, et al. Cav2.3 channels contribute to dopaminergic neuron loss in a model of Parkinson’s disease. Nat Commun, 2019,10(1):5094 - DOI
-
- Rice RA, Berchtold NC, Cotman CW, et al. Age-related downregulation of the CaV3.1 T-type calcium channel as a mediator of amyloid beta production. Neurobiol Aging, 2014,35(5):1002–1011 - DOI
MeSH terms
Substances
Supplementary concepts
LinkOut - more resources
Full Text Sources
Molecular Biology Databases