Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 May 11:12:58-70.
doi: 10.7150/jgen.93243. eCollection 2024.

The impact of Elaeagnus angustifolia root exudates on Parafrankia soli NRRL B-16219 exoproteome

Ikram Kammoun  1 , Guylaine Miotello  2 , Karim Ben Slama  3 , Jean Armengaud  2 , Faten Ghodhbane-Gtari  1   4 , Maher Gtari  1
Affiliations

The impact of Elaeagnus angustifolia root exudates on Parafrankia soli NRRL B-16219 exoproteome

Ikram Kammoun et al. J Genomics. .

Abstract

Root exudates from host plant species are known to play a critical role in the establishment and maintenance of symbiotic relationships with soil bacteria. In this study, we investigated the impact of root exudates from compatible host plant species; Elaeagnus angustifolia on the exoproteome of Parafrankia soli strain NRRL B-16219. A total of 565 proteins were evidenced as differentially abundant, with 32 upregulated and 533 downregulated in presence of the plant exudates. Analysis of the function of these proteins suggests that the bacterial strain is undergoing a complex metabolic reprogramming towards a new developmental phase elicited in presence of host plant root exudates. The upregulation of Type II/IV secretion system proteins among the differentially expressed proteins indicates their possible role in infecting the host plant, as shown for some rhizobia. Additionally, EF-Tu, proteins upregulated in this study, may function as an effector for the T4SSs and trigger plant defense responses. These findings suggest that Parafrankia soli may use EF-Tu to infect the actinorhizal host plant and pave the way for further investigations of the molecular mechanisms underlying the establishment of symbiotic relationships.

PubMed Disclaimer

Conflict of interest statement

Competing Interests: The authors have declared that no competing interest exists.

Figures

Figure 1
Figure 1
Experimental pipeline used in the present study for root exudate production, treatment of NRRL B-16219, protein precipitation and NanoLC-MS/MS analysis.
Figure 2
Figure 2
COG classification of significantly affected proteins in Parafrankia soli strain NRRL B-16219 exoproteome in presence of root exudates of E. angustifolia host species. A graphical representation of up- (a) and down-expressed proteins (p value ≤ 0.05, Tfold ≥ 1.5 and hits/class score = 9 (0-9, 0 being not significant, ranked based on Benjamini-Hochberg Algorithm)).
Figure 2
Figure 2
COG classification of significantly affected proteins in Parafrankia soli strain NRRL B-16219 exoproteome in presence of root exudates of E. angustifolia host species. A graphical representation of up- (a) and down-expressed proteins (p value ≤ 0.05, Tfold ≥ 1.5 and hits/class score = 9 (0-9, 0 being not significant, ranked based on Benjamini-Hochberg Algorithm)).
Figure 3
Figure 3
GO terms significantly affected in Parafrankia soli strain NRRL B-16219 exoproteome in presence of root exudates of E. angustifolia host species. A graphical representation of up- (a) and down-expressed proteins (p value ≤ 0.05, Tfold ≥ 1.5 and hits/class score = 9 (0-9, 0 being not significant, ranked based on Benjamini-Hochberg Algorithm)). Only hits/class size >2 are presented.
Figure 4
Figure 4
KEGG terms significantly affected in Parafrankia soli strain NRRL B-16219 exoproteome in presence of root exudates of E. angustifolia host species. A graphical representation of down-expressed proteins (p value ≤ 0.05, Tfold ≥ 1.5 and hits/class score = 9 (0-9, 0 being not significant, ranked based on Benjamini-Hochberg Algorithm)).
See this image and copyright information in PMC

Similar articles

See all similar articles

References

    1. Gtari M. Taxogenomic status of phylogenetically distant Frankia clusters warrants their elevation to the rank of genus: A description of Protofrankia gen. nov, Parafrankia gen. nov, and Pseudofrankia gen. nov. as three novel genera within the family Frankiaceae. Front Microbiol. 2022;13:1041425. - PMC - PubMed
    1. Oren A, Göker M. Validation List no. 210. Valid publication of new names and new combinations effectively published outside the IJSEM. Int J Syst Evol Microbiol. 2023;73(3):1–6. - PubMed
    1. Venturi V, Keel C. Signaling in the rhizosphere. Trends Plant Sci. 2016;21(3):187–198. - PubMed
    1. Pawlowski K, Bisseling T. Rhizobial and actinorhizal symbioses: what are the shared features? Plant Cell. 1996;8(10):1899. - PMC - PubMed
    1. Brewin NJ. Plant cell wall remodelling in the Rhizobium-legume symbiosis. Crit Rev Plant Sci. 2004;23(4):293–316.

LinkOut - more resources

-