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DOI: 10.1055/s-0035-1556131
Global lipidomics profiling of cotton seed oil genotypes using CO2-based chromatography coupled to mass spectrometry
Refined cottonseed oil enjoys widespread applications in the food and chemical industries. Although the major lipids comprising cottonseed oil are well known, there are many diverse lipid species in cotton seeds that occur at much lower levels and have important nutritional or anti-nutritional properties. Lipidomics analysis of complex lipids in seed oil extracts using a single chromatographic technique is challenging due to the diversity of lipid polarity and the large range of concentrations of lipid species in biological samples. Ultra Performance Convergence Chromatography (UPC2) is a chromatographic system that utilizes liquid CO2 as primary solvent and co-solvents such as methanol as a mobile phase to leverage the chromatographic principles and selectivity of normal phase chromatography while providing the ease-of-use of reversed-phase LC. We have used UPC2 coupled to mass spectrometry for the separation of free fatty acids, neutral and polar lipids in a single cotton seed lipid extract. Several seed oil genotypes, including high oleic acid line of Gossypium hirsutum cv. Coker 312 with a nonfunctional Brassica napus allele of delta-12 fatty acid desaturase (FAD2) and naturally occurring high oleic acid line of G. barbadense, were profiled to identify global consequences of modulating seed oil fatty acid composition. Potential markers that discriminate the different genotypes were identified and quantified.