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DOI: 10.1055/s-0035-1556314
Studies on the exploitation of Broussonetia papyrifera and its endophytic fungi
Broussonetia papyrifera (L.) Vent.(Moraceae) is a deciduous tree which is native to Asia. Its fructus is the common TCM “Chushizi” with bioactivities. Its leaves and barks also have medicinal uses. The endophytic fungi from B. papyrifera provide new natural products with biological activities. A total of 47 strains were isolated from the different parts of the tree. The leaves have high islation rates (average 0.91) with rich biodiversity. The anti-inflammatory activity of the fermentation products was investigated with mice macrophages RAW 264.7 in vitro. The results indicated that 14 endophytes could fully inhibit the growth of inflammatory cell at the concentration of 100 µg/mL. The cytotoxic activity of fermentation products was evaluated by the MTT method, and five endophytic fungi possess significant anti-hepatoma activity in human SMMC-7721 and QJY7023 cell lines. Thirty-six compounds were isolated from the ethyl acetate extract of the two strains Chaetomium globousm and Alternaria sp. Altertoxin IV is a new compound. Altersolanol A showed significant inhibition to human MG-63 and SMMC-7721 cell lines with IC50 values of 0.53 and 2.92 µg/mL. Endophytic fungi of Broussonetia papyrifera are promising sources of natural bioactive metabolites.
