Induction of G1 arrest and differentiation in MDA-MB-231 breast cancer cell by boehmeriasin A, a novel compound from plant
- PMID: 16445628
- DOI: 10.1111/j.1525-1438.2006.00291.x
Induction of G1 arrest and differentiation in MDA-MB-231 breast cancer cell by boehmeriasin A, a novel compound from plant
Abstract
Boehmeriasin A is a new phenanthroquinolizidine alkaloid recently isolated from the Boehmeria siamensis Craib (Urticaceae). In vitro biological activity assay demonstrated that this novel compound has wide-range, strong antitumor activity. This study is aimed to determine the effects of boehmeriasin A on breast cancer cell (MDA-MB-231 cell line). Proliferation assay and fluorescence activated cell sorter (FACS) showed that cell growth inhibition and G1 phase arrest of cell cycle were caused by boehmeriasin A. The concentrations resulting in total and 50% growth inhibition are 0.007 and 0.0035 microg/mL, respectively. Exposed in 0.007 microg/mL boehmeriasin A for 12 h, the G1 phase cell percent increased from 44.8% pre-drug treatment to 66.3%. Consistent with G1 arrest and cell growth inhibition, cyclin E2 and cyclin D1 messenger RNA expression in the cell was down-regulated with drug treatment. Then, few apoptotic cells were detected, and most other cells underwent differentiation, which is characterized by specific changes in cell morphology, lots of lipid droplet accumulation, and increasing expression of adipocyte differentiation-related protein. The result first demonstrates that boehmeriasin A potently inhibits the proliferation of breast cancer cell MDA-MB-231 via the G1 phase cell cycle arrest and differentiation induction, and as such, may be considered as candidate chemotherapeutic and/or chemopreventive agent for breast cancer.
Similar articles
-
Insulin-like growth factor binding protein-3 induces G1 cell cycle arrest with inhibition of cyclin-dependent kinase 2 and 4 in MCF-7 human breast cancer cells.Horm Metab Res. 2010 Mar;42(3):165-72. doi: 10.1055/s-0029-1243190. Epub 2009 Dec 3. Horm Metab Res. 2010. PMID: 19960406
-
A polyphenolic fraction from grape seeds causes irreversible growth inhibition of breast carcinoma MDA-MB468 cells by inhibiting mitogen-activated protein kinases activation and inducing G1 arrest and differentiation.Clin Cancer Res. 2000 Jul;6(7):2921-30. Clin Cancer Res. 2000. PMID: 10914742
-
Daidzein causes cell cycle arrest at the G1 and G2/M phases in human breast cancer MCF-7 and MDA-MB-453 cells.Phytomedicine. 2008 Sep;15(9):683-90. doi: 10.1016/j.phymed.2008.04.006. Epub 2008 Jun 9. Phytomedicine. 2008. PMID: 18541420
-
Induction of G1 phase arrest and apoptosis in MDA-MB-231 breast cancer cells by troglitazone, a synthetic peroxisome proliferator-activated receptor gamma (PPARgamma) ligand.Cell Biol Int. 2008 Aug;32(8):906-12. doi: 10.1016/j.cellbi.2008.04.011. Epub 2008 Apr 10. Cell Biol Int. 2008. PMID: 18474441
-
Saucernetin-7 isolated from Saururus chinensis inhibits proliferation of human promyelocytic HL-60 leukemia cells via G0/G1 phase arrest and induction of differentiation.Carcinogenesis. 2004 Aug;25(8):1387-94. doi: 10.1093/carcin/bgh143. Epub 2004 Mar 19. Carcinogenesis. 2004. PMID: 15033903
Cited by
-
Induction of G1-phase cell cycle arrest and apoptosis pathway in MDA-MB-231 human breast cancer cells by sulfated polysaccharide extracted from Laurencia papillosa.Cancer Cell Int. 2016 May 26;16:39. doi: 10.1186/s12935-016-0315-4. eCollection 2016. Cancer Cell Int. 2016. PMID: 27231438 Free PMC article.
-
Morusin suppresses breast cancer cell growth in vitro and in vivo through C/EBPβ and PPARγ mediated lipoapoptosis.J Exp Clin Cancer Res. 2015 Nov 4;34:137. doi: 10.1186/s13046-015-0252-4. J Exp Clin Cancer Res. 2015. PMID: 26538209 Free PMC article.
-
Enrichment and characterization of cancer stem‑like cells from a cervical cancer cell line.Mol Med Rep. 2014 Jun;9(6):2117-23. doi: 10.3892/mmr.2014.2063. Epub 2014 Mar 20. Mol Med Rep. 2014. PMID: 24676900 Free PMC article.
-
Anti-hepatitis B virus activity of Boehmeria nivea leaf extracts in human HepG2.2.15 cells.Biomed Rep. 2014 Jan;2(1):147-151. doi: 10.3892/br.2013.205. Epub 2013 Nov 22. Biomed Rep. 2014. PMID: 24649087 Free PMC article.
-
Total Syntheses and Cytotoxicity of (R)- and (S)-Boehmeriasin A.ACS Med Chem Lett. 2011 Apr 14;2(4):313-315. doi: 10.1021/ml1003074. ACS Med Chem Lett. 2011. PMID: 21779519 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical
Research Materials
Miscellaneous