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. 2006 May;3(5):333-41.
doi: 10.1016/j.cmet.2006.04.002.

Complementary action of the PGC-1 coactivators in mitochondrial biogenesis and brown fat differentiation

Marc Uldry  1 , Wenli Yang, Julie St-Pierre, Jiandie Lin, Patrick Seale, Bruce M Spiegelman
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Free article

Complementary action of the PGC-1 coactivators in mitochondrial biogenesis and brown fat differentiation

Marc Uldry et al. Cell Metab. 2006 May.
Free article

Abstract

Mitochondria play an essential role in the ability of brown fat to generate heat, and the PGC-1 coactivators control several aspects of mitochondrial biogenesis. To investigate their specific roles in brown fat cells, we generated immortal preadipocyte lines from the brown adipose tissue of mice lacking PGC-1alpha. We could then efficiently knockdown PGC-1beta expression by shRNA expression. Loss of PGC-1alpha did not alter brown fat differentiation but severely reduced the induction of thermogenic genes. Cells deficient in either PGC-1alpha or PGC-1beta coactivators showed a small decrease in the differentiation-dependant program of mitochondrial biogenesis and respiration; however, this increase in mitochondrial number and function was totally abolished during brown fat differentiation when both PGC-1alpha and PGC-1beta were deficient. These data show that PGC-1alpha is essential for brown fat thermogenesis but not brown fat differentiation, and the PGC-1 coactivators play an absolutely essential but complementary function in differentiation-induced mitochondrial biogenesis.

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