Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 2008 Apr;99(4):659-67.
doi: 10.1160/TH07-08-0525.

Prolonged in-vivo half-life of factor VIIa by fusion to albumin

Thomas Weimer  1 , Wilfried Wormsbächer, Ulrich Kronthaler, Wiegand Lang, Uwe Liebing, Stefan Schulte
Affiliations
Comparative Study

Prolonged in-vivo half-life of factor VIIa by fusion to albumin

Thomas Weimer et al. Thromb Haemost. 2008 Apr.

Abstract

For the treatment of haemophilia patients with inhibitors, recombinant factor VIIa (rFVIIa) is available as a therapeutic option to control bleeding episodes with a good balance of safety and efficacy. However, the short in-vivo half-life of approximately 2.5 hours makes multiple injections necessary, which is inconvenient for both physicians and patients. Here we describe the generation of a recombinant FVIIa molecule with an extended half-life based on genetic fusion to human albumin. The recombinant FVII albumin fusion protein (rVII-FP) was expressed in mammalian cells and upon activation displayed a FVII activity close to that of wild type FVIIa. Pharmacokinetic studies in rats demonstrated that the half-life of the activated recombinant FVII albumin fusion protein (rVIIa-FP) was extended six- to seven-fold compared with wild type rFVIIa. The in-vitro and in-vivo efficacy was evaluated and was found to be comparable to a commercially available rFVIIa (NovoSeven((R))). The results of this study demonstrate that it is feasible to develop a half-life extended FVIIa molecule with haemostatic properties very similar to the wild-type factor.

PubMed Disclaimer

Comment in

  • Factor VIIa gets even bigger.
    Sheffield WP, Clarke BJ. Sheffield WP, et al. Thromb Haemost. 2008 Apr;99(4):653-4. doi: 10.1160/TH08-02-0120. Thromb Haemost. 2008. PMID: 18392320 No abstract available.

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

Publication types

MeSH terms

LinkOut - more resources

-