Effects of In Vitro Digestion on the Content and Biological Activity of Polyphenols from Acacia mearnsii Bark

doi:10.3390/molecules23071804

. 2018 Jul 20;23(7):1804.

doi: 10.3390/molecules23071804.

Effects of In Vitro Digestion on the Content and Biological Activity of Polyphenols from Acacia mearnsii Bark

Xiao Chen¹, Jia Xiong², Lingxiao He³, Yu Zhang⁴, Xun Li⁵, Liping Zhang⁶, Fei Wang⁷

Affiliations

¹ Jiangsu Key Laboratory for the Chemistry and Utilization of Agro-Forest Biomass, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China. 13770765711@163.com.
² Plants for Human Health Institute, Food Bioprocessing and Nutrition Sciences Department, North Carolina State University, North Carolina Research Campus, Kannapolis, NC 28081, USA. jxiong5@ncsu.edu.
³ Jiangsu Key Laboratory for the Chemistry and Utilization of Agro-Forest Biomass, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China. a6410@126.com.
⁴ Jiangsu Key Laboratory for the Chemistry and Utilization of Agro-Forest Biomass, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China. yuzhang@njfu.edu.cn.
⁵ Jiangsu Key Laboratory for the Chemistry and Utilization of Agro-Forest Biomass, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China. xunlee@163.com.
⁶ College of Materials Sciences and Technology, Beijing Forestry University, Beijing 100083, China. zhanglp418@163.com.
⁷ Jiangsu Key Laboratory for the Chemistry and Utilization of Agro-Forest Biomass, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China. hgwf@njfu.edu.cn.

PMID: 30037047
PMCID: PMC6100292
DOI: 10.3390/molecules23071804

Effects of In Vitro Digestion on the Content and Biological Activity of Polyphenols from Acacia mearnsii Bark

Xiao Chen et al. Molecules. 2018.

. 2018 Jul 20;23(7):1804.

doi: 10.3390/molecules23071804.

Authors

Xiao Chen¹, Jia Xiong², Lingxiao He³, Yu Zhang⁴, Xun Li⁵, Liping Zhang⁶, Fei Wang⁷

Affiliations

¹ Jiangsu Key Laboratory for the Chemistry and Utilization of Agro-Forest Biomass, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China. 13770765711@163.com.
² Plants for Human Health Institute, Food Bioprocessing and Nutrition Sciences Department, North Carolina State University, North Carolina Research Campus, Kannapolis, NC 28081, USA. jxiong5@ncsu.edu.
³ Jiangsu Key Laboratory for the Chemistry and Utilization of Agro-Forest Biomass, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China. a6410@126.com.
⁴ Jiangsu Key Laboratory for the Chemistry and Utilization of Agro-Forest Biomass, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China. yuzhang@njfu.edu.cn.
⁵ Jiangsu Key Laboratory for the Chemistry and Utilization of Agro-Forest Biomass, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China. xunlee@163.com.
⁶ College of Materials Sciences and Technology, Beijing Forestry University, Beijing 100083, China. zhanglp418@163.com.
⁷ Jiangsu Key Laboratory for the Chemistry and Utilization of Agro-Forest Biomass, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China. hgwf@njfu.edu.cn.

PMID: 30037047
PMCID: PMC6100292
DOI: 10.3390/molecules23071804

Abstract

The stability and bioaccessibility of polyphenol from Acacia mearnsii bark were measured at various stages during in vitro simulated digestion. Subsequently, the changes in the total polyphenol content (TPC) and biological activity were studied. The results showed that the phenolic compounds from A. mearnsii remained stable, and TPC underwent few changes during gastric digestion. Nonetheless, intestinal digestion led to the degradation of proanthocyanidins (PAs) and a significant decrease in TPC (26%). Degradation was determined by normal-phase HPLC and gel permeation chromatography. Only monomers, dimers, and trimers of flavan-3-ols were identified in the serum-accessible fraction for characterization of their bioaccessibility. The results also indicated the obvious antioxidant capacity of PAs from A. mearnsii bark, and ~53% of the α-glucosidase⁻inhibitory effect was preserved. All these findings show that PAs from A. mearnsii bark as a native plant source may be particularly beneficial for human health as a natural nutritional supplement.

Keywords: Acacia mearnsii; HPLC/MS; antidiabetic; antioxidant; in vitro digestion; proanthocyanidins.

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Conflict of interest statement

There are no potential conflicts of interest to be reported by the authors.

Figures

**Figure 1**
Representative normal-phase HPLC/VWD chromatograms of samples from *A. mearnsii* bark at different time points during the simulated gastric-intestinal digestion, (A) gastric digestion; (B) intestinal digestion. The OUT samples represent the solution outside the tubing.

**Figure 2**
Representative gel permeation chromatograms of the raw extract, gastric digestion at 120 min, and OUT 120 min samples prepared in tetrahydrofuran.

**Figure 3**
Percent α-glucosidase inhibition for gastric digesta and OUT samples at different time points during in vitro gastrointestinal digestion. The mean values reported at each digestion stage-those with a common superscript (a–c) are not significantly different from each other (p < 0.05). Data are means of three independent determinations ± SD.

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References

1. Aron P.M., Kennedy J.A. Flavan-3-ols: Nature, occurrence and biological activity. Mol. Nutr. Food Res. 2008;52:79–104. doi: 10.1002/mnfr.200700137. - DOI - PubMed
1. Venter P.B., Senekal N.D., Kemp G., Amra-Jordaan M., Khan P., Bonnet S.L., Westhuizen J.H.V.D. Analysis of commercial proanthocyanidins. Part 3: The chemical composition of wattle (Acacia mearnsii) bark extract. Phytochemistry. 2012;83:153–167. doi: 10.1016/j.phytochem.2012.07.012. - DOI - PubMed
1. Xiong J., Graceb M.H., Esposito D., Wang F., Lila M.A. Phytochemical characterization and anti-inflammatory properties of Acacia mearnsii leaves. Nat. Prod. Commun. 2016;11:649–653. - PubMed
1. Gey K.F. The antioxidant hypothesis of cardiovascular disease: Epidemiology and mechanisms. Biochem. Soc. Trans. 1990;18:1041–1045. doi: 10.1042/bst0181041. - DOI - PubMed
1. Ness A.R., Powles J.W. Fruit and vegetables, and cardiovascular disease: A review. Int. J. Epidemiol. 1997;26:1–13. doi: 10.1093/ije/26.1.1. - DOI - PubMed

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[1] Aron P.M., Kennedy J.A. Flavan-3-ols: Nature, occurrence and biological activity. Mol. Nutr. Food Res. 2008;52:79–104. doi: 10.1002/mnfr.200700137. - DOI - PubMed

[2] Aron P.M., Kennedy J.A. Flavan-3-ols: Nature, occurrence and biological activity. Mol. Nutr. Food Res. 2008;52:79–104. doi: 10.1002/mnfr.200700137. - DOI - PubMed

[3] Venter P.B., Senekal N.D., Kemp G., Amra-Jordaan M., Khan P., Bonnet S.L., Westhuizen J.H.V.D. Analysis of commercial proanthocyanidins. Part 3: The chemical composition of wattle (Acacia mearnsii) bark extract. Phytochemistry. 2012;83:153–167. doi: 10.1016/j.phytochem.2012.07.012. - DOI - PubMed

[4] Venter P.B., Senekal N.D., Kemp G., Amra-Jordaan M., Khan P., Bonnet S.L., Westhuizen J.H.V.D. Analysis of commercial proanthocyanidins. Part 3: The chemical composition of wattle (Acacia mearnsii) bark extract. Phytochemistry. 2012;83:153–167. doi: 10.1016/j.phytochem.2012.07.012. - DOI - PubMed

[5] Xiong J., Graceb M.H., Esposito D., Wang F., Lila M.A. Phytochemical characterization and anti-inflammatory properties of Acacia mearnsii leaves. Nat. Prod. Commun. 2016;11:649–653. - PubMed

[6] Xiong J., Graceb M.H., Esposito D., Wang F., Lila M.A. Phytochemical characterization and anti-inflammatory properties of Acacia mearnsii leaves. Nat. Prod. Commun. 2016;11:649–653. - PubMed

[7] Gey K.F. The antioxidant hypothesis of cardiovascular disease: Epidemiology and mechanisms. Biochem. Soc. Trans. 1990;18:1041–1045. doi: 10.1042/bst0181041. - DOI - PubMed

[8] Gey K.F. The antioxidant hypothesis of cardiovascular disease: Epidemiology and mechanisms. Biochem. Soc. Trans. 1990;18:1041–1045. doi: 10.1042/bst0181041. - DOI - PubMed

[9] Ness A.R., Powles J.W. Fruit and vegetables, and cardiovascular disease: A review. Int. J. Epidemiol. 1997;26:1–13. doi: 10.1093/ije/26.1.1. - DOI - PubMed

[10] Ness A.R., Powles J.W. Fruit and vegetables, and cardiovascular disease: A review. Int. J. Epidemiol. 1997;26:1–13. doi: 10.1093/ije/26.1.1. - DOI - PubMed

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Effects of In Vitro Digestion on the Content and Biological Activity of Polyphenols from Acacia mearnsii Bark

Affiliations

Effects of In Vitro Digestion on the Content and Biological Activity of Polyphenols from Acacia mearnsii Bark

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Conflict of interest statement

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