Rabbit and human atherosclerotic lesions contain IgG that recognizes epitopes of oxidized LDL
- PMID: 7506053
- DOI: 10.1161/01.atv.14.1.32
Rabbit and human atherosclerotic lesions contain IgG that recognizes epitopes of oxidized LDL
Abstract
Atherosclerotic lesions contain relatively large quantities of IgG. We have previously shown that both human and rabbit sera contain autoantibodies against epitopes of oxidized (Ox) low-density lipoprotein (LDL) and that LDL isolated from atherosclerotic lesions contains small amounts of tightly bound IgG. However, it is not known whether IgG isolated from atherosclerotic lesions recognizes epitopes present in native LDL or Ox-LDL. IgG was isolated from Watanabe heritable hyperlipidemic (WHHL) rabbit atherosclerotic lesions by sequential salt extractions, purified by fast protein liquid chromatography on protein G, and used in a solid-phase radioimmunoassay. IgG and immune complexes were also isolated from the saline extracts of human lesions by adsorption onto latex beads coated with anti-human IgG antibodies or protein A. IgG isolated from rabbit lesions showed significant titers against malondialdehyde (MDA)-modified LDL and LDL oxidized by copper ions for 4 and 18 hours but not against native LDL. On Western blots, lesion IgG stained MDA-LDL and fragments of Ox-LDL. Western blots of immune complexes isolated from human lesions revealed the presence in the isolated complexes of both apoprotein B and apoprotein B fragments, which reacted with antibodies to MDA-lysine. Furthermore, rabbit lesion IgG immunostained epitopes of Ox-LDL present in human atherosclerotic lesions. Immunostains obtained with rabbit lesion IgG were similar to those obtained with a monoclonal antibody specific for MDA-lysine. The results show that human and rabbit atherosclerotic lesions contain IgG that recognizes epitopes characteristic of Ox-LDL. These data suggest that immunologic processes may be an important component of the atherogenic process.
Similar articles
-
Human-derived anti-oxidized LDL autoantibody blocks uptake of oxidized LDL by macrophages and localizes to atherosclerotic lesions in vivo.Arterioscler Thromb Vasc Biol. 2001 Aug;21(8):1333-9. doi: 10.1161/hq0801.093587. Arterioscler Thromb Vasc Biol. 2001. PMID: 11498462
-
Radiolabeled MDA2, an oxidation-specific, monoclonal antibody, identifies native atherosclerotic lesions in vivo.J Nucl Cardiol. 1999 Jan-Feb;6(1 Pt 1):41-53. doi: 10.1016/s1071-3581(99)90064-8. J Nucl Cardiol. 1999. PMID: 10070840
-
Low density lipoprotein oxidation and atherogenesis: from experimental models to clinical studies.G Ital Cardiol. 1997 Dec;27(12):1302-14. G Ital Cardiol. 1997. PMID: 9470066 Review.
-
Cloning of monoclonal autoantibodies to epitopes of oxidized lipoproteins from apolipoprotein E-deficient mice. Demonstration of epitopes of oxidized low density lipoprotein in human plasma.J Clin Invest. 1996 Aug 1;98(3):800-14. doi: 10.1172/JCI118853. J Clin Invest. 1996. PMID: 8698873 Free PMC article.
-
Macrophages and oxidized low density lipoproteins in the pathogenesis of atherosclerosis.Ann Med. 1991;23(5):561-7. doi: 10.3109/07853899109150518. Ann Med. 1991. PMID: 1721825 Review.
Cited by
-
Cytokine Profiling of Plasma and Atherosclerotic Plaques in Patients Undergoing Carotid Endarterectomy.Int J Mol Sci. 2024 Jan 14;25(2):1030. doi: 10.3390/ijms25021030. Int J Mol Sci. 2024. PMID: 38256102 Free PMC article.
-
Impact of Immunity on Coronary Artery Disease: An Updated Pathogenic Interplay and Potential Therapeutic Strategies.Life (Basel). 2023 Oct 27;13(11):2128. doi: 10.3390/life13112128. Life (Basel). 2023. PMID: 38004268 Free PMC article. Review.
-
B cell-specific knockout of AID protects against atherosclerosis.Sci Rep. 2023 May 30;13(1):8723. doi: 10.1038/s41598-023-35980-1. Sci Rep. 2023. PMID: 37253865 Free PMC article.
-
Mapping the functions of IgM antibodies in atherosclerotic cardiovascular disease.Nat Rev Cardiol. 2023 Jul;20(7):433-434. doi: 10.1038/s41569-023-00888-w. Nat Rev Cardiol. 2023. PMID: 37169831 No abstract available.
-
Modified Lipoproteins Induce Arterial Wall Inflammation During Atherogenesis.Front Cardiovasc Med. 2022 Mar 3;9:841545. doi: 10.3389/fcvm.2022.841545. eCollection 2022. Front Cardiovasc Med. 2022. PMID: 35310965 Free PMC article. Review.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources